Primary Rat Annulus Fibrosus Cells (AFC)

CSI008Ra01

Primary Rat Annulus Fibrosus Cells (AFC)

1628 €

CSI008Mu01

Primary Mouse Annulus Fibrosus Cells (AFC)

1598 €

ABC-TC3489

Human Annulus Fibrosus Cells

Degeneration of the intervertebral disk is a major pathological process implicated in low back pain. The causes of low back pain have been suggested to involve genetic, environmental, and psychosocial factors. The anulus fibrosus makes up the peripheral portion of disk structure, composed of fibrocartilage and type I and II collagen. Cells of the intervertebral disc are influenced by both biophysical and mechanical factors in their local environment. The anulus fibrosus cells can be stimulated by interleukin 1 beta to produce factors implicated in local degradative and inflammatory processes. Although mechanical stress is an important modulator of the degeneration, the underlying molecular mechanism remains unclear. The human annulus firosus cell culture provides an in vitro model for the study of cellular and molecular events involved in disc degeneration, tissue engineering and cell therapy for spine disc disorders.HAFC from Gentaur Research Laboratories are isolated from annulus fibrosus of human intervertibral disc. HAFC are cryopreserved at primary or passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HAFC are characterized by immunofluorescent method with antibodies to fibronectin and vimentin. HAFC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAFC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.

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ABC-TC5508

Aged Rat Primary Aortic Endothelial Cells

Aged Rat Primary Aortic Endothelial Cells from Gentaur are isolated from aorta of 74 week old laboratory Sprague-Dawley rat. Aged Rat Primary Aortic Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x106 cells per ml and is delivered frozen. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 (CD31) antibody, following the application of magnetic beads pre-coated with secondary antibody.

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CSI138Ra01

Primary Rat Ovarian Fibroblasts (OF)

1493 €

T4862

Rat Primary Intestinal Smooth Muscle Cells

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