PoASMCs-RFP cells are selected from RFP-lentiviral transfected PoASMCs resistant to PUROMYCIN. The cells are shipped in a frozen vial(the cells are provided @ passage 3). Smooth Muscle Cells(SMCs) Growth Medium is recommended for the expansion of PoASMCs-RFP and these cells can be propagated to extra 3-4 passages without losing their morphologic and phenotypic characteristics when cultured following the detailed protocol described below.
Human Gastric Carcinoma N87 Cells (N87 cells) were established from the liver metastatic site of a male gastric carcinoma patient. RFP-Tagged Human Gastric Carcinoma N87 Cells (RFP-N87 cells) are selected from N87 cells after infected with lentiviruses expressing RFP with BSD. The cells can be cultivated with Universal Full Growth Medium and they have a minimum population doubling capacity > 12 when cultured following the detailed protocol described below.