Catalog number: 500 - SC065-LR
Product Category: Business & Industrial > Science & Laboratory
Size: 2 x 106 cell/ml x 1ml
SC065-LR
Luciferase & RFP expression stable cell line in Mouse MB49 cells with Puromycin resistance
SC065-R
RFP expression stable cell line in Mouse MB49 cells with Puromycin resistance
SC065-LG
Luciferase & GFP expression stable cell line in Mouse MB49 cells with Puromycin resistance
ABC-FC0045
HNPCs are initiated by dissecting fetal brain tissue and digestion with collagenase. RFP expressing HNPCs (RFP-HNPCs) are selected from RFP expressing lentiviruses infected HNPCs. RFP-HNPCs are offered in frozen vial format (the cells are provided @ passage 2). RFP-HNPCs growth medium is recommended for cell culture and these cells have a minimum average population doubling capacity 6 when cultured following the detailed protocol described below.
ABC-FC0046
Human dopaminergic neuronal cells are isolated from fetal brain tissue obtained from agencies authorized to procure and distribute tissues for research. Dopaminergic neuronal cells are selected in tyrosine free medium supplemented with a mixture of growth factors. Actively growing population of cells are tested for tyrosine hydroxylase (TH) expression by immunocytochemistry. RFP-Expressing Human dopaminergic neuronal cells (RFP-HDaNCs) are selected by puromycin after HDaNCs are infected with lentiviruses expressing RFP. Cells are provided with more than 5x10[5] cells/ vial at passage 2. The cells are grown in regular tissue culture dishes and subcultured at a split ratio of 1 to 2 at confluence. We recommend not to use the cells beyond 4 passages. There may be a significant decrease in the number of tyrosine hydroxylase positive cells after 4 passages as they are not maintained in selective medium to allow the growth of only cells expressing tyrosine hydroxylase.
ABC-FC0050
RFP-bAECs are selected from bAECs transfected using lentivirus expressing RFP using Puromycin. The cells are shipped in a frozen vial(the cells are provided @ passage 2). Endothelial Growth Medium is recommended for the expansion of RFP-bAECs and these cells can be propagated to at least 12 population doubling times without losing their morphologic and phenotypic characteristics when cultured following the detailed protocol described below.