ABC-TC3909

Monkey Liver Sinusoidal Endothelial Cells

Monkey Liver Sinusoidal Endothelial Cells from Gentaur are isolated from tissue of Cynomolgus Monkey. Monkey Liver Sinusoidal Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.Monkey Liver Sinusoidal Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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ABC-TC4154

Rat Liver Sinusoidal Endothelial Cells

Rat Liver Sinusoidal Endothelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal Sprague-Dawley Rats. Rat Liver Sinusoidal Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.Rat Liver Sinusoidal Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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ABC-TC3679

Human Liver Sinusoidal Endothelial Cells

Endothelial cells play multiple physiological functions and are central to many pathological processes. The liver contains two distinct endothelial cell types: vascular and sinusoidal. Sinusoidal endothelial cells (SEC) are microvascular endothelial cells with a unique phenotype reminiscent of dendritic cells and a unique function as antigen-presenting cells for CD4+ T cells. Thus, SEC represent a new type of organ-resident "non-professional" antigen-presenting cell that appears to be involved in the local control of the immune response and the induction of immune tolerance in the liver. The hepatic microenvironment, i.e. portal venous constituents and soluble mediators from sinusoidal cell populations, tightly control antigen presentation by SEC to avoid immune-mediated damage. SEC express well-characterized surface receptors and differ morphologically and metabolically from large-vessel endothelia. It has reported that SEC are dynamic regulators of porosity that respond rapidly and locally to environmental zonal stimuli during liver regeneration. Due to its strategic position in the liver sinusoid, SEC dysfunction and structural alterations have far-reaching repercussions for the whole liver. HHSEC from Gentaur Research Laboratories are isolated from human liver. HHSEC are cryopreserved immediately after purification and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HHSEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM). HHSEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HHSEC are guaranteed to further expand for 15 population doublings in the conditions provided by Gentaur Research Laboratories.

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GWB-5D3E85

ENDOTHELIAL CELLS, Antibody

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GWB-841A35

Endothelial Cells Antibody

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ABC-HP030X

Rat Liver Kupffer Cells

Rat Liver Kupffer Cells are isolated from the liver of 4-6 week old Sprague-Dawley rat. Rat Liver Kupffer Cells are cryopreserved at passage 0 and delivered frozen. Cells are tested for expression of markers using antibodies, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.

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