Monkey Intestinal Fibroblasts
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Catalog number: 870 - ABC-TC3906
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1 vial
ABC-TC3655
Human Intestinal Fibroblasts
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. For example, the development of bowl stenosis in Crohn’s disease patients is caused by extreme fibroblast proliferation and extracellular matrix expansion.HIF from Gentaur Research Laboratories are isolated from human intestinal tissue. HIF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HIF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HIF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HIF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.
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ABC-TC3906
Monkey Intestinal Fibroblasts
Monkey Primary Intestinal Fibroblasts from Gentaur are isolated from tissue of Cynomolgus Monkey. Monkey Primary Intestinal Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Monkey Primary Intestinal Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
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ABC-TC3929
Mouse Intestinal Fibroblasts
Mouse intestinal fibroblasts, 2-week C57B mouse
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ABC-TC4055
Rabbit Aortic Fibroblasts
Rabbit Primary Aortic Fibroblasts from Gentaur are isolated from aortic tissue of New Zealand White Rabbits. Rabbit Primary Aortic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Rabbit Primary Aortic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.