Luciferase(firefly) Hela Report Cell Line(Blasticidin)
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Catalog number: 870 - ABC-RC146D
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1 vial
ABC-RC099D
GAS(Luc) Report Cell Line-HeLa
The GAS reporter (Luc)-HeLa cell line is designed to monitor the activity of IFN gamma-induced signal transduction pathways in cultured cells by measuring activated STAT1 homodimers. It contains a firefly luciferase gene driven by three copies of the IFN gamma-activated sites (GAS) located upstream of the minimal TATA promoter. IFNγ first binds to a heterodimeric receptor consisting of two chains, IFNGR1 and IFNGR2, causing its dimerization and the activation of specific Janus family kinases (JAK1 and JAK2). Two STAT1 molecules associate with this ligand-activated receptor complex and are activated by phosphorylation to form active homodimer. The active STAT1 homodimers translocate to the nucleus where they bind IFN gamma-activated sites (GAS) in the promoter of IFNγ inducible genes, including luciferase reporter gene.
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ABC-RC146D
Luciferase(firefly) Hela Report Cell Line(Blasticidin)
Transformed from Hela cells by lentiviral system with sequence verified firefly luciferase gene. Luciferase was expressed under CMV promoter, A Blasticidin antibiotic marker was expressed under Rsv promoter. The cell constitutively express Luciferase enzyme.
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ABC-RC147D
Luciferase(firefly) Hela Report Cell Line(Puromycin)
Transformed from Hela cells by lentiviral system with sequence verified firefly luciferase gene. Luciferase was expressed under CMV promoter, A Puromycin antibiotic marker was expressed under Rsv promoter. The cell constitutively express Luciferase enzyme.
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78161
Cas9-Expressing HeLa Cell Pool
Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing HeLa cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.
1355.92 €
LYSATE0023
Human Hela Whole Cell Lysate
This cell lysate is prepared from human hela using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
424.20 €