Luciferase-2A-GFP(Puromycin) Report Cell Line-A459
Ask for quotation
On request
quantity
product details
Catalog number: 870 - ABC-RC131D
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1 vial
ABC-RC131D
Luciferase-2A-GFP(Puromycin) Report Cell Line-A459
Transformed from huamn lung cancer cell line, A549 by lentiviral system with firefly luciferase gene II and GFP. The Luciferase and GFP was co-expressed under CMV promoter as seperated protein(not as fusion). The Puromycin marker was expressed under Rsv promoter. The cell constitutively co-expresses both firefly Luciferase and GFP. Each cell demonstrates strong GFP fluorescent signal and luciferase assay signal(Note: this is pooled stable ell population, not originated from a single cell colony. Thus you will observe the uneven GFP signal intensity amount cells.
Ask for quotation
ABC-RC130D
Luciferase(Puromycin) Report Cell Line-A459
Transformed from huamn lung cancer cell line, A549 by lentiviral system with firefly luciferase gene. The Luciferase was under modified CMV promoter. The Puromycin marker was expressed under Rsv promoter. The cell line demonstrates high level of bioluminescence signal via firefly luciferase assay.
Ask for quotation
ABC-RC150D
Luciferase-2A-GFP Report Cell Line(Blasticidin)-Hela
Transformed from Hela cells by lentiviral system with sequence verified firefly luciferase gene II and GFP. The Luciferase and GFP was co-expressed under CMV promoter as seperated protein(not as fusion). The Blasticidin marker was expressed under Rsv promoter.
Ask for quotation
ABC-RC128D
loxP-GFP-RFP(Neo) Report Cell Line-HEK293
The CRE reporting cell lines are used to monitor or confirm the efficiency of CRE recombination in vivo. It is a great method and easy tool to verify the performance of your CRE enzyme(your CRE expression plasmids, or pre-made CRE expression lentivirus, or purified CRE enzyme) in vivo conditions. It is also a control test to verify your CRE-loxP based system. Transformed from HEK293 cells, expressing "LoxP-GFP-stop-LoxP-RFP" cassette under CMV promoter(see Color-Switch cassette below). Cell line also contains a Neomycin antibiotic marker under RSV promoter.The cell line demonstrates strong GFP fluorescent signal in normal culture condition as the constitutive CMV promoter drives the GFP high expression to the stop codon. The downstream RFP ORF was not expressed because of the stop codon after the GFP ORF. Once the CRE protein was present in nuclear, the CRE excises/deletes the DNA fragment between two loxP sites, which removes the stop codon(see the DNA structure scheme above). As a result, the RFP ORF is then expressed under the CMV promoter, and the cell line switches to RFP fluorescent. The RFP signal can be easily monitored via fluorescent cell sorting(for the ratio between GFP and RFP cells), or visualized under microscope, or measured the fluorescent intensity by a meter or reader with RFP filter set.
Ask for quotation
ABC-RC129D
loxP-GFP-RFP(Puro) Report Cell Line-HEK293
The CRE reporting cell lines are used to monitor or confirm the efficiency of CRE recombination in vivo. It is a great method and easy tool to verify the performance of your CRE enzyme(your CRE expression plasmids, or pre-made CRE expression lentivirus, or purified CRE enzyme) in vivo conditions. It is also a control test to verify your CRE-loxP based system. Transformed from HEK293 cells, expressing "LoxP-GFP-stop-LoxP-RFP" cassette under CMV promoter(see Color-Switch cassette below). Cell line also contains a Puromycin antibiotic marker under RSV promoter.The cell line demonstrates strong GFP fluorescent signal in normal culture condition as the constitutive CMV promoter drives the GFP high expression to the stop codon. The downstream RFP ORF was not expressed because of the stop codon after the GFP ORF. Once the CRE protein was present in nuclear, the CRE excises/deletes the DNA fragment between two loxP sites, which removes the stop codon(see the DNA structure scheme above). As a result, the RFP ORF is then expressed under the CMV promoter, and the cell line switches to RFP fluorescent. The RFP signal can be easily monitored via fluorescent cell sorting(for the ratio between GFP and RFP cells), or visualized under microscope, or measured the fluorescent intensity by a meter or reader with RFP filter set.
Ask for quotation
ABC-RC133D
Luciferase/GFP Report Cell Line-Human B lymphocyte
Transformed from huamn B lymphocyte cell line(from 61 years male), the Luciferase and GFP was constitutively co-expressed under EF1a promoter as two seperated protein(not as fusion), mediated via 2A element. The Puromycin resistance marker was expressed under Rsv promoter. Each cell demonstrates strong GFP fluorescent signal and luciferase assay signal via Luc assay(Note: It is the pooled stable cell population, not originated from a single cell colony. Thus you will observe the uneven GFP signal intensity amount cells)