
Katalognummer: 1140 - 5100
Produktkategori: Företag och industri > Vetenskap och laboratorium
Storlek: 5 Plate
| Storage and shipping | Insulated Cooler, Overnight |
|---|
5000
A rapid and simple two-color assay to discriminate between live and dead cells. Compatible with fluorescence microscopy, flow cytometry, and plate reader assays.Live: Ex 495 nm / Em 515 nm | Dead: Ex 528 nm / Em 617 nmION Vital - Viability kit can be used to quantify live and dead cells within eukaryotic cell suspensions or adherent cultures, 3D cultures, organoids, and some non-fixed tissues. Cannot be used for yeast or bacteria. When following our protocol, the Viability kit provides enough reagents to make 150 mL of working solution, enough for fifteen 96-well plates or 1,500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations for your application.Calcein AM is a membrane-permeant, non-fluorescent form of calcein that enters cells passively. Once inside the cytosol of cells, Calcein AM is converted to green fluorescent Calcein by ubiquitous esterases in viable cells, resulting in uniform cytosolic fluorescence (Ex/Em 495 nm/515 nm). Calcein, a polyanionic dye, is membrane-impermeant and is well retained within the cytosol of most healthy cells with intact cell membranes. Ethidium homodimer I (EthD-I) is a membrane-impermeable, high-affinity, nucleic acid stain that is excluded by viable cells with intact cell membranes. When membrane integrity is compromised, a hallmark of dead or dying cells, EthD-I enters the cell and binds DNA, which results in a >30-fold enhancement in bright red, nuclear fluorescence (Ex/Em 528 nm/617 nm).
1497527
No wash, ratiometric calcium flux assay, 2 plates. Total assay solution compatible with plate reader and fluorescence microscopy applications. Excitation: 340, 380 nm | Emission: 505 nm | Kd: 145 nMRatiometric Calcium Essentials kit provides the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give the user the flexibility needed to customize your assay.
6000-10
No wash, ratiometric calcium flux assay, 10 plates. Total assay solution compatible with plate reader and fluorescence microscopy applications. Excitation: 340, 380 nm | Emission: 505 nm | Kd: 145 nMRatiometric Calcium Essentials kit provides the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give the user the flexibility needed to customize your assay.
11000-10
No wash thallium assay, 10 plates. Total assay solution for plate-based, high-throughput measurements of intracellular thallium flux. Also useful for potassium and sodium channels, cation transporters, and GPCRs. Excitation: 490 nm | Emission: 515 nmION Biosciences' Brilliant Thallium (Tl⁺) Assay is a total assay solution for multi-well plate-based, high-throughput measurements of Tl⁺ flux through K⁺, Na⁺, non-selective cation channels, and some Na⁺ or K⁺ transporters. The ION Biosciences' Brilliant Thallium Assay is also useful for a wide range of effectors of ion channels and transporters including G protein-coupled receptors, lipid and protein kinase, for example. In multi-well, plate-based formats, the Brilliant Thallium Assay can be used to discover and characterize the effects of tens to many tens-of-thousands of compounds and environmental factors on effectors of Tl⁺ flux. Over the past 15 years, fluorescence-based measures of Tl⁺ flux have been widely utilized to discover small molecule modulators of a host of ion channels, transporters, GPCRS and other targets of interest for both drug discovery and basic research.
11000-100
No wash thallium assay, 100 plates. Total assay solution for plate-based, high-throughput measurements of intracellular thallium flux. Also useful for potassium and sodium channels, cation transporters, and GPCRs.Excitation: 490 nm | Emission: 515 nmION Biosciences' Brilliant Thallium (Tl⁺) Assay is a total assay solution for multi-well plate-based, high-throughput measurements of Tl⁺ flux through K⁺, Na⁺, non-selective cation channels, and some Na⁺ or K⁺ transporters. The ION Biosciences' Brilliant Thallium Assay is also useful for a wide range of effectors of ion channels and transporters including G protein-coupled receptors, lipid and protein kinase, for example. In multi-well, plate-based formats, the Brilliant Thallium Assay can be used to discover and characterize the effects of tens to many tens-of-thousands of compounds and environmental factors on effectors of Tl⁺ flux. Over the past 15 years, fluorescence-based measures of Tl⁺ flux have been widely utilized to discover small molecule modulators of a host of ion channels, transporters, GPCRS and other targets of interest for both drug discovery and basic research.
11000-2
No wash thallium assay, 2 plates. Total assay solution for plate-based, high-throughput measurements of intracellular thallium flux. Also useful for potassium and sodium channels, cation transporters, and GPCRs. Excitation: 490 nm | Emission: 515 nmION Biosciences' Brilliant Thallium (Tl⁺) Assay is a total assay solution for multi-well plate-based, high-throughput measurements of Tl⁺ flux through K⁺, Na⁺, non-selective cation channels, and some Na⁺ or K⁺ transporters. The ION Biosciences' Brilliant Thallium Assay is also useful for a wide range of effectors of ion channels and transporters including G protein-coupled receptors, lipid and protein kinase, for example. In multi-well, plate-based formats, the Brilliant Thallium Assay can be used to discover and characterize the effects of tens to many tens-of-thousands of compounds and environmental factors on effectors of Tl⁺ flux. Over the past 15 years, fluorescence-based measures of Tl⁺ flux have been widely utilized to discover small molecule modulators of a host of ion channels, transporters, GPCRS and other targets of interest for both drug discovery and basic research.

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