IDE Inhibitor Screening Assay Kit BPS Bioscience

IDE Inhibitor Screening Assay Kit

0.01 EURAsk for quotation

On request

quantity

Produktdetaljer

Katalognummer: 421 - 78883-2

Produktkategori: Företag och industri > Vetenskap och laboratorium

Leverantör:

BPS BioscienceGentaur

Storlek: 384 Reactions

Parameters

Additional information	Applications: Screen small molecule inhibitors in high throughput screening (HTS) applications.;
Storage and shipping	Shipping conditions: -80°C; Storage conditions: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.;
Storage and shipping	With

78883-1

IDE Inhibitor Screening Assay Kit

The IDE (Insulin-degrading enzyme) Inhibitor Screening Assay is a homogeneous fluorogenic assay designed to measure the activity of IDE for screening and profiling applications. The IDE assay kit comes in a convenient 96-well or 384-well format and contains enough purified IDE (amino acids 71-254), substrate, and PR-02 buffer for 100 or 400 reactions.Figure 1: Illustration of the assay principle.The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.Need us to run inhibitor screens or profile your compounds against IDE? Check out ourProtease Screening Services.

Ask for quotation

HY-12655

IDE-IN-1

IDE-IN-1 is an insulin-degrading enzyme (IDE) inhibitor with IC50 value of 50 nM. IDE-IN-1 can be used to study type 2 diabetes[1].

Ask for quotation

E-EL-H0429-24Tests

Human IDE-Insulin Degrading Enzyme- ELISA Kit

Sandwich

13838 €

78840

USP20 Inhibitor Screening Assay Kit

The USP20 Inhibitor Screening Assay Kit is a 96-well format fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme USP20 for screening and profiling applications. To determine the effect of an inhibitor on USP20 activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases.Figure 1: Illustration of the assay principle.Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC) . In the conjugated form, the energy emitted from fluorochrome AMC is quenched. Upon proteolysis, AMC is no longer quenched and emits fluorescence with λexcitation/ λemission maxima of 350/460 nm. The increase in fluorescence is proportional to the DUB activity.Need us to run inhibitor screens or profile your compounds against USP20? Check out ourProtease Screening ServicesorUbiquitination Screening Services.

Ask for quotation

78859

USP2 Inhibitor Screening Assay Kit

The Ubiquitin specific peptidase 2 (USP2) Inhibitor Screening Assay Kit is a homogeneous fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme USP2 for screening and profiling applications. The kit contains enough purified USP2 recombinant protein, Ubiquitinated-AMC substrate, and assay buffer for 100 reactions.To determine the effect of an inhibitor on USP2 activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases.Figure 1: Illustration of the assay principle.Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC) . In the conjugated form, the energy emitted from fluorochrome AMC is quenched. Upon proteolysis, AMC is no longer quenched and emits fluorescence with an λexcitation/ λemission maxima of 350/460 nm. The increase in fluorescence is proportional to the DUB activity.Need us to run inhibitor screens or profile your compounds against USP2? Check out ourProtease Screening ServicesorUbiquitination Screening Services.

Ask for quotation

78865

USP14 Inhibitor Screening Assay Kit

The USP14 Inhibitor Screening Assay Kit is a 96-well fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme USP14 (Ubiquitin specific peptidase 14) for screening and profiling applications. The kit contains enough purified USP14 protein, Ubiquitinated-AMC substrate, and assay buffer for 100 reactions.To determine the effect of an inhibitor on USP14 activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases.Figure 1: Illustration of the assay principle.Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC) . In conjugated form, the energy emitted from the fluorochrome AMC is quenched. Upon proteolysis, AMC is no longer quenched and emits fluorescence with λexcitation/λemission maxima of 350/460 nm. The increase in fluorescence is proportional to the DUB activity.Need us to run inhibitor screens or profile your compounds against USP14? Check out ourProtease Screening ServicesorUbiquitination Screening Services.

Ask for quotation

Håll dig uppdaterad! Visa tidigare publikationer

By: Author , 2 Comment