Catalog number: 870 - ABC-TC3839
Product Category: Business & Industrial > Science & Laboratory
Size: 1 vial
ABC-TC4233
Rat Primary Tracheal Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Primary Tracheal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Primary Tracheal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Primary Tracheal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
ABC-TC3839
The epithelium is the interface between the body and the external environment and covers all exterior surfaces and interior lumina. Depending on the tissue of origin, the functions of epithelial cells are diverse and include absorption, secretion, protection, transcellular transport, and sensation.Gentaur offers a range of Epithelia Cells produced at Gentaur's cell culture facility. The cells are isolated from normal human adult tissues of the airways (nasal mucosa, trachea, bronchi,and distal respiratory tract), breast (mammary gland), kidney (whole kidney and renal cortex), and the placenta (amniotic membrane).Shortly after isolation, all Gentaur Human Epithelial Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium,Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.
ABC-TC3448
Human Bronchial/Tracheal Epithelial Cells, when grown in LIBro Medium, provide an ideal serum-free culture model for the accurate studies of toxicity, cystic fibrosis, asthma, pathogenesis, pharmacology or airway wound healing. HBTEC are cultured without retinoic acid and cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HBTEC are quality tested in LIBro B/T Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media. Cell Features:HBTEC are cryopreserved as primary cells. Cells are isolated from human bronchi/trachea and expanded once in culture vessels before being harvested for cryopreservation.HSAEC are cryopreserved after primary cells are isolated from human lung tissue and expanded once in culture vessels before being harvested for cryopreservation. Human Airway Epithelial Cells are not exposed to retinoic acid during isolation or expansion.Human Airway Epithelial Cells are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality.
ABC-TC4116
Rat Corneal Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Corneal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Corneal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Corneal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
ABC-TC4118
Rat Dermal Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Dermal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Dermal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Dermal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
ABC-TC4133
Rat Primary Esophageal Epithelial Cells from Gentaur are isolated from tissue of adult laboratory Sprague–Dawley Rats. Rat Primary Esophageal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Primary Esophageal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Primary Esophageal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.