Human Subclavian Artery Endothelial Cells (HScAEC ) are derived from normal human subclavian arteries. They are cryopreserved at second passage and can be cultured and propagated at least 10 population doublings.
GFP-HAAECs were selected from HAAECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 14 when cultured following the detailed protocol described below.
GFP-HCAECs were selected from HCAECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 14 when cultured following the detailed protocol described below.