NPB-BDCA4+ Lymphoid/Plasmacytoid Dendritic cells have been positively isolated from mononuclear cells using immunomagnetic BDCA-4 MicroBead labeling system.
Primary human immature dendritic cells (DCs) were derived from immunomagnetically selected peripheral blood (PB) monocytes. Monocytes were cultured in RPMI 1640 Medium with 10% fetal bovine serum (FBS), GM-CSF and IL-4 for 5 days to generate immature DCs. PB was collected using one of the following anticoagulants: acid-citrate-dextrose (ACD), acid-citrate-dextrose solution A (ACDA), citrate-phosphate-dextrose (CPD), citrate-phosphate-double-dextrose (CP2D), or citrate-phosphate-dextrose-adenine (CPDA).
Included Diagnoses: Adenocarcinoma (including all subtypes) and Squamous Cell Carcinoma (including all subtypes),andnbsp, Neuroendocrine tumors of the Kidney, Renal Cell Carcinoma, Clear Cell Carcinoma and other carcinomas of the kidney, Urothelial tumors of the kidney.
Included Diagnoses: Adenocarcinoma (including all subtypes) and Squamous Cell Carcinoma (including all subtypes), Cholangiocarcinoma, Neuroendocrine tumors of the liver, and Hepatocellular carcinoma.
Included Diagnoses: Adenocarcinoma (including all subtypes) and Squamous Cell Carcinoma (including all subtypes), Neuroendocrine tumors of the esophagus, Carcinoma of the esophagus.
NPB-BDCA4+ Lymphoid/Plasmacytoid Dendritic cells have been positively isolated from mononuclear cells using immunomagnetic BDCA-4 MicroBead labeling system.