Human Intestinal Epithelial Cells
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Catalog number: 870 - ABC-TC3879
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1 vial
ABC-TC3879
Human Intestinal Epithelial Cells
Cryopreserved InEpC are truly primary cells representing both villi (enterocytes, goblet, and enteroendocrine cells) and crypts structures. In combination with human intestinal myofibroblasts (InMyoFib).
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ABC-TC4140
Rat Intestinal Epithelial Cells
Rat Intestinal Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Intestinal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Intestinal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Intestinal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
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ABC-TC5510
Porcine Small Intestinal Epithelial Cells
Porcine Primary Small Intestinal Epithelial Cells from Gentaur are isolated from small intestinal tissue of porcine. Porcine Primary Small Intestinal Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.
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ABC-TC3612
Human Esophageal Epithelial Cells
The human esophagus is lined by a non-keratinizing, moist stratified squamous epithelium whose apical cell membranes and intercellular junctional complexes combine to produce an effective permeability barrier against the influx of luminal content. In particular, the barrier created by these structures limits exposure of the surface cells' basolateral cell membranes and entire membrane of cells of the deeper layers to the wide swings in osmolality occurring regularly within the esophageal lumen. Histologically, the esophageal epithelium consists of two zones, the basal and differentiated zones. Cellular proliferation is limited to the basal zone, and cells are thought to migrate from this area towards the esophageal lumen. Migration is associated with the initiation of differentiation and the sequential expression of differentiation markers. The availability of human esophageal epithelial cell culture provides an excellent in vitro model in the study of the physiology of esophageal epithelium and the mechanisms of the esophageal carcinogenesis.HEEC from Gentaur Research Laboratories are isolated from the human esophagus. HEEC are cryopreserved on passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HEEC are characterized by immunofluorescent method with antibodies to cytokeratine-8, -18 and -19. HEEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEEC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.
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ABC-TC3669
Human Lens Epithelial Cells
Human Lens Epithelial Cells from Gentaur Research Laboratories are isolated from the human lens. HLEpiC are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HLEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, cytokeratin-19 and fibronectin. HLEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLEpiC are guaranteed to further culture in the conditions provided by Gentaur Research Laboratories.
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ABC-TC3719
Human Nasal Epithelial Cells
The cells are isolated from normal human adult tissues of the airways (nasal mucosa, trachea, bronchi, and distal respiratory tract), breast (mammary gland), kidney (whole kidney and renal cortex), and the placenta (amniotic membrane). Shortly after isolation, all Gentaur Human Epithelial Cells are cryopreserved at passage 2 (P2) . Each cryo vial contains more than 500,000 viable cells after thawing. Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.