60618

Hippo Pathway TEAD Luciferase Reporter MCF7 cell line

The TEAD Reporter - MCF7 cell line contains the firefly luciferase gene under the control of TEAD responsive elements stably integrated into the human breast cancer cell line, MCF7. Inside the cells, basal unphosphorylated YAP/TAZ remains in the nucleus and induces the constitutive expression of luciferase reporter. The cell line is validated for the inhibition of the expression of luciferase reporter by the activators of the Hippo pathway.

3855.68 €

ABC-RC0094

Human Hippo Pathway TEAD Reporter Cell Line-MCF7

Human Hippo Pathway TEAD Reporter Cell Line is derived from MCF7. It can be used for monitoring Hippo pathway activity and screen for activators or inhibitors of the Hippo pathway.

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ABC-RC116D

Hippo Pathway TEAD Report Cell Line-MCF7

The TEAD Reporter andndash; MCF7 cell line contains the firefly luciferase gene under the control of TEAD responsive elements stably integrated into the human breast cancer cell line, MCF7. Inside the cells, basal unphosphorylated YAP/TAZ remains in the nucleus and induces the constitutive expression of luciferase reporter. The cell line is validated for the inhibition of the expression of luciferase reporter by the activators of the Hippo pathway.

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MBS154205-1Pack

MCF7 Cell Slide

437.96 €

MBS154205-5x1Pack

MCF7 Cell Slide

1275.00 €

79503

Notch1 Pathway Reporter Kit (Human)

Notch Pathway Reporter kit is designed for monitoring the activity of the Notch signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for Human NOTCH1 that has a deletion of the entire extracellular domain, leaving the transmembrane and intracellular domains intact (Notch1DE). Inside the cells, the NOTCH1 DE is cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_This kit contains the expression vector for Human Notch1DE. We also offer the Mouse Notch1DE expression vector (BPS Bioscience #60509), as well as a Mouse Notch1 NICD expression vector, which is constitutively active without processing by γ-secretase (BPS Bioscience #79504)._x000D_

1136.25 €