ABC-TC3613

Human Esophageal Fibroblasts

Esophageal fibroblasts which line the lamina propria of the upper and lower esophagus are mesenchymal cells that originated from the embryonic mesoderm. They have been shown to play an important role in angiogenesis through TGF-β and VEGF signaling pathways in the highly angiogenic esophageal squamous cell carcinoma. Predominantly, fibroblasts secrete a nonrigid extracellular matrix that is both rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in the connective tissues and play major roles in wound healing. Because they are one of the simplest types of cells to grow in culture fibroblasts have been extensively used for a wide range of cellular and molecular studies. In addition, fibroblasts’ durability makes them amenable to a wide variety of manipulations ranging from gene transfection to microinjection.The HEF cells from Gentaur Research Laboratories are isolated from human esophageal tissue. HEF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HEF are characterized by immunofluorescence with antibody specific to fibronectin. HEF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEF are guaranteed to further expand for 15 population doublings at the conditions provided by Gentaur Research Laboratories.

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T4232

Human Primary Esophageal Fibroblasts

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CSC-I2202Z

Immortalized Mouse Esophageal Fibroblasts-SV40

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ABC-TC3910

Monkey Lymphatic Fibroblasts

Monkey Primary Lymphatic Fibroblasts from Gentaur are isolated from tissue of Cynomolgus Monkey. Monkey Primary Lymphatic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Monkey Primary Lymphatic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

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ABC-TC3912

Mouse Aorta Fibroblasts

Mouse aorta fibroblasts, 2-week ICR mouse

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ABC-TC3929

Mouse Intestinal Fibroblasts

Mouse intestinal fibroblasts, 2-week C57B mouse

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