Human Epidermal Keratinocytes, Adult, Pooled
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Catalog number: 870 - ABC-TC3976
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1 vial
ABC-TC3976
Human Epidermal Keratinocytes, Adult, Pooled
Epidermal keratinocytes represent the major cell type of the epidermis, making up about 90% of the cells. They originate in the stratum basale and undergo gradual differentiation including profound morphological changes during their shift to the stratum corneum. In the stratum corneum, the final barrierlayer of the skin, keratinocytes are found as nucleus-free, flat, and highly keratinized squamous cells. Gentaur offers a range of Normal Human Epidermal Keratinocytes (NHEK) from single donors or pooled donors produced at Promo- Cell’s cell culture facility. The cells are isolated from juvenile foreskin or from adult normal human tissue from different locations, e.g. face, breast, abdomen, and thighs. Shortly after isolation, all Gentaur Normal Human Epidermal Keratinocytes are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing. Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.
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ABC-TC3961
Human Epidermal Keratinocytes, Neonatal, Pooled
Normal Human Epidermal Keratinocytes (NHEK) have longer guaranteed life spans than KGM or KGM-2 cells with ≥18 total population doublings for Adult cells and ≥20 population doublings for neonatal cells.
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ABC-TC3604
Human Epidermal Keratinocytes, Adult
The epithelial layer of the skin provides an essential function as a protective barrier against insult from the outside environment. The major cell type in this layer is keratinocytes which consists around 85% of living epidermal cells. Keratinocytes are the cells of stratified squamous epithelia. It is so named because its most abundant protein is keratin. Keratinocyte divide and are thus generated in the basal layer of the epidermis; this division is followed by a programmed death as they produce keratin and move up to the surface of the epidermis. Keratinocyte proliferation, differentiation, and apoptosis are complex and carefully choreographed processes. Besides the protective function, keratinocytes also play a central role in tissue homeostasis, wound healing, cancers, and skin-based gene-therapy. Human keratinocytes express adhesion molecules and cytokines indicating their participation in skin innate immunity and homeostasis in vivo. HEK-a from Gentaur Research Laboratories are isolated from adult human epidermal tissue. HEK-a are cryopreserved on primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HEK-a are characterized by immunofluorescent method with antibodies to cytokeratine-18 and -19. HEK-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEK-a are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.