Human Dermal Fibroblasts, Neonatal
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Catalog number: 870 - ABC-TC3451
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1 vial
ABC-TC4123
Rat Dermal Fibroblasts-neonatal
Rat Primary Dermal Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Dermal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Dermal Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
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ABC-TC3451
Human Dermal Fibroblasts, Neonatal
Human Dermal Fibroblasts-Neonatal are isolated form neonatal human skin. Human Dermal Fibroblasts-Neonatal are cryopreserved at P1 and delivered frozen. Human Dermal Fibroblasts-Neonatal are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. Human Dermal Fibroblasts-Neonatal are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
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