Human Brain Vascular Smooth Muscle Cells
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Catalog number: 870 - ABC-TC3527
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 1 vial
ABC-TC3527
Human Brain Vascular Smooth Muscle Cells
Vascular smooth muscle cell is the cellular substate of most significant arterial disease. The increased growth potential of vascular smooth muscle cells represents one of the crucial anomalies responsible for the development of essential vascular diseases. New studies demonstrate that vascular smooth muscle cells express ICAM-1 and VCAM-1, which may contribute to the inflammatory reaction in the vascular wall and may actively be involved in the progression and stability of vascular disease. In vitro culture of human VSMC as a model of vascular research played a critical role and continues providing information in the pharmacology and the therapy of vascular diseases.HBVSMC from Gentaur Research Laboratories are isolated from human brain. HBVSMC are cryopreserved at secondary culture after purification and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HBVSMC are characterized by immunofluorescent method with antibodies to andALPHA;-smooth muscle actin and desmin. HBVSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBVSMC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.
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ABC-TC4101
Rat brain vascular smooth muscle cells
Rat brain vascular smooth muscle cells
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ABC-TC3176
C57BL/6 Mouse Brain Vascular Smooth Muscle Cells
Mouse Brain Vascular Smooth Muscle Cells from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Brain Vascular Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Brain Vascular Smooth Muscle Cells are characterized by immunofluorescent cell staining with antibodies of α-smooth muscle actin and are negative for bacteria, yeast, fungi, and mycoplasma.Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur.Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
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ABC-TC3514
Human Bladder Smooth Muscle Cells
Smooth muscle tissue is found in the tunica media layer of large and small blood vessels and in the walls of hollow organs like the bladder and the uterus. Smooth Muscle Cells (SMC) possess all the same types of filaments, but, depending on the tissue of origin, differ significantly in mechanical and physiological properties. Therefore, Gentaur offers a range of Human Smooth Muscle Cells produced at Gentaur's cell culture facility from normal human tissue of different origins. The cells are isolated from the aorta, the coronary artery, the pulmonary artery, the umbilical artery,the trachea, the bronchi, the bladder,the prostate, and the uterus. Shortly after isolation, all Gentaur Human Smooth Muscle Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.
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ABC-TC3530
Human Bronchial Smooth Muscle Cells
Human Bronchial Smooth Muscle Cells from Gentaur Research Laboratories are isolated from human bronchi and bronchioles. HBSMC are cryopreserved at secondary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HBSMC are characterized by immunofluorescent method with antibodies to smooth muscle actin and desmin. HBSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBSMC are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.
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ABC-TC3572
Human Colonic Smooth Muscle Cells
Smooth muscle contraction is the fundamental event in gastrointestinal motion. Inflammation of the human intestine causes thickening of the smooth muscle layers which results from the increases in the smooth muscle-specific actins. The increased smooth muscle actins may affect force production and further demonstrate the plasticity of smooth muscle in the inflamed intestine. Human intestinal smooth muscle cells respond to IL-1-beta and TNF-alpha stimulation by releasing IL-6, which might significantly contribute to the overall systemic inflammatory response. The colonic smooth muscle cells possess a different subtype of somatostatin receptor from gastric smooth muscle cells. The availability of human gastrointestinal smooth muscle cells in culture will considerably enhance our ability to study the contractile, proliferative and connective tissue responses of the smooth muscle of the human gastrointestinal tract.HCSMC from Gentaur Research Laboratories are isolated from the human colon. HCSMC are cryopreserved at primary or passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCSMC are characterized by immunofluorescent method with antibodies to andALPHA;-smooth muscle actin and desmin. HCSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCSMC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.