GFP-HLiSMVECs were selected from HRMVECs resistant to puromycin after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 4-5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 16 when cultured following the detailed protocol described below.
RFP-HLiSMVECs were selected from HRMVECs resistant to Zeocin after infected with RFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 4-5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 16 when cultured following the detailed protocol described below.
Bovine Brain Microvascular Endothelial Cells (BBMVEC) are isolated from microvessels of bovine brain. The cells are cryopreserved at second passage. BBMVEC can be cultured and propagated for at least 12 population doublings.