Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under enhanced EF1a promoter with RFP-Puromycin fluorescent-antibioic fusion dual marker. Particles was pellet down and re-suspended in PBS solution.
Pre-made optional inducible lentiviral shRNA expression particles containing a hairpin insert to knockdown human P53 gene. It also contains a RFP-puromycin fusion marker under Rsv promoter. Virus was concentrated and provided in PBS solution.
Pre-made lentiviral particles bicistronically express individual firefly luciferase and nuclear permeable CRE recombinase under the same suCMV promoter. A RFP-Puromycin fusion dual marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.