Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under enhanced EF1a promoter with RFP-Puromycin fluorescent-antibioic fusion dual marker. Particles was pellet down and re-suspended in PBS solution.
Pre-made lentiviral particles bicistronically express individual firefly luciferase and nuclear permeable CRE recombinase under the same suCMV promoter. A RFP-Puromycin fusion dual marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.
Pre-made lentiviral particles for sub-cellular fluorescent labeling, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. RFP fluorescent signal targeted in Nuclear Membrane by the inner nuclear membrane localization signal from lamin B membrane receptor.