CMV-ACE2-GFP (Puro) Lentiviral Particles

vAP-0100

CMV-ACE2-GFP (Puro) Lentiviral Particles

879 €

LCV-0026-2S

CMV-ACVR1C-GFP-Puro

Human ACVR2B ORF cDNA Lentivirus. Lentiviral particles, packaged with expression vector for human ACVR2B (uniprot ID Q8NER5). A CMV promoter drives expression of the ORF fused to a C-terminal epitope tag, and fluorescent reporter, which is separated by a self-cleaving peptide.These lentiviral particles allow for easy transduction and high stable expression of the indicated ORF cDNA in mammalian cells. The lentiviral vectors have been optimized to provide high gene expression and reliable genome integration, allowing for easy establishment of long-term, stable cell lines with consistent read out of fluorescent (GFP, RFP, BFP) or luminescent (firefly luc) reporters. Transcription of the ORF cDNA fused with C-terminal epitope tag is controlled by either EF1a or CMV promoter, while expression of the reporter or selection marker (puromycin or blasticidin) is separated by a self-cleaving peptide to allow separate translation via the same mRNA transcript. In some constructs, the CMV or EF1a promoter drives expression of cDNA-Tag, while the GFP/RFP reporter and drug selection marker are driven by PGK promoter and separated by a self-cleaving peptide. The particles are capable of transducing a variety of difficult-to-transfect cells, including primary and/or thawed cells, making them an extremely versatile product for studying genes in mammalian systems. The accuracy of ORF cDNA cloning has been confirmed by sequencing and the successful protein expression has been validated by transient transfection.

595 €

LCV-0027-2S

CMV-ACVR2B-GFP-Puro

Human ACVR2B ORF cDNA Lentivirus. Lentiviral particles, packaged with expression vector for human ACVR2B (uniprot ID Q13705). A CMV promoter drives expression of the ORF fused to a C-terminal epitope tag, and fluorescent reporter, which is separated by a self-cleaving peptide.These lentiviral particles allow for easy transduction and high stable expression of the indicated ORF cDNA in mammalian cells. The lentiviral vectors have been optimized to provide high gene expression and reliable genome integration, allowing for easy establishment of long-term, stable cell lines with consistent read out of fluorescent (GFP, RFP, BFP) or luminescent (firefly luc) reporters. Transcription of the ORF cDNA fused with C-terminal epitope tag is controlled by either EF1a or CMV promoter, while expression of the reporter or selection marker (puromycin or blasticidin) is separated by a self-cleaving peptide to allow separate translation via the same mRNA transcript. In some constructs, the CMV or EF1a promoter drives expression of cDNA-Tag, while the GFP/RFP reporter and drug selection marker are driven by PGK promoter and separated by a self-cleaving peptide. The particles are capable of transducing a variety of difficult-to-transfect cells, including primary and/or thawed cells, making them an extremely versatile product for studying genes in mammalian systems. The accuracy of ORF cDNA cloning has been confirmed by sequencing and the successful protein expression has been validated by transient transfection.

595 €

LVP579

GFP (CAG)-Puro lentiviral particles 

Pre-made lentiviral particles expressing GFP under the CAG promoter with Puromycin selection marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

439.75 €

LVP800

GFP (TetCMV-Puro) lentiviral particles

Pre-made lentiviral particles expressing eGFP with Puromycin antibiotic marker, provided in DMEM medium with 10% FBS.

439.75 €

LVP812-P

CD44CR1-GFP (Puro) Lentiviral particles 

Pre-made lentiviral particles with GFP reporter under CD44 promoter. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

649.75 €