Katalognummer: 870 - ABC-TC5473
Produktkategori: Företag och industri > Vetenskap och laboratorium
Storlek: 1 vial
ABC-TC5473
BKS db Mouse Bladder Microvascular Endothelial Cells are isolated from the bladder microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Bladder Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
ABC-TC3513
The walls of capillaries are composed of a single layer of microvascular endothe-lial cells. These cells differ in morphology and other properties depending on the tissues the capillaries supply. Therefore, Gentaur offers a range of Microvascular Endothelial Cells produced at Gentaur's cell culture facility from normal human tissues of different origins.Shortly after isolation, all Gentaur Microvascular Endothelial Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.