Transformed from huamn lung cancer cell line, A549 by lentiviral system with firefly luciferase gene II and GFP. The Luciferase and GFP was co-expressed under CMV promoter as seperated protein(not as fusion). The Puromycin marker was expressed under Rsv promoter. The cell constitutively co-expresses both firefly Luciferase and GFP. Each cell demonstrates strong GFP fluorescent signal and luciferase assay signal(Note: this is pooled stable ell population, not originated from a single cell colony. Thus you will observe the uneven GFP signal intensity amount cells.
Transformed from Hela cells by lentiviral system with sequence verified firefly luciferase gene II and GFP. The Luciferase and GFP was co-expressed under CMV promoter as seperated protein(not as fusion). The Puromycin marker was expressed under Rsv promoter.