Yellow fever virus E protein ectodomain（YFV-E）
401.98 €
In Stock
quantity
product details
Catalog number: 1144 - NCP0146
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 100ug
NCP0146
Yellow fever virus E protein ectodomain（YFV-E）
Yellow fever virus is a flavivirus that causes the disease yellow fever. The virus is found in tropical South America and sub-Saharan Africa, and is maintained in a primate-mosquito transmission cycle. The disease varies from a febrile illness to a lethal haemorrhagic fever. There is no antiviral treatment but there is a very effective live attenuated vaccine, strain 17D.
401.98 €
NCP0151
Omsk hemorrhagic fever virus E protein ectodomain（OHFV-E）
Omsk hemorrhagic fever virus (OHFV) is a tick-borne flavivirus classified as a biosafety level-4 (BSL4) pathogen. Studies of OHFV are restricted to be conducted within BSL4 laboratories. Currently, no commercial vaccines or antiviral drugs are available against OHFV infection. In this study, we recovered a replication-deficient OHFV with an NS1 deletion (OHFV-ΔNS1) and reporter virus replacing NS1 with the Gaussia luciferase (Gluc) (OHFV-ΔNS1-Gluc). Both the defective OHFV-ΔNS1 and OHFV-ΔNS1-Gluc virus could only replicate efficiently in the BHK21 cell line expressing NS1 (BHK21NS1) but not in naïve BHK21 cells. The Gluc reporter gene of OHFV-ΔNS1-Gluc virus was maintained stably after serial passaging of BHK21NS1 cells and was used to surrogate the replication of OHFV. Using NITD008, OHFV-ΔNS1-Gluc virus was validated for antiviral screening, and high-throughput screening parameters were optimized in a 96-well plate format with a calculated Z' value above 0.5. The OHFV-ΔNS1-Gluc reporter virus is a powerful tool for antiviral screening as well as viral replication and pathogenesis studies in BSL2 laboratories.
401.98 €