USP8 Inhibitor Screening Assay Kit BPS Bioscience

USP8 Inhibitor Screening Assay Kit

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quantity

Produktdetaljer

Katalognummer: 421 - 79998

Produktkategori: Företag och industri > Vetenskap och laboratorium

Leverantör:

BPS BioscienceGentaur

Storlek: 96 Reactions

Parameters

Additional information	Applications: Study enzyme kinetics and screening small molecule inhibitors for drug discovery and high-throughput screening (HTS) applications.;
Storage and shipping	Shipping conditions: -80°C; Storage conditions: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.;
Storage and shipping	With

78840

USP20 Inhibitor Screening Assay Kit

The USP20 Inhibitor Screening Assay Kit is a 96-well format fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme USP20 for screening and profiling applications. To determine the effect of an inhibitor on USP20 activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases.Figure 1: Illustration of the assay principle.Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC) . In the conjugated form, the energy emitted from fluorochrome AMC is quenched. Upon proteolysis, AMC is no longer quenched and emits fluorescence with λexcitation/ λemission maxima of 350/460 nm. The increase in fluorescence is proportional to the DUB activity.Need us to run inhibitor screens or profile your compounds against USP20? Check out ourProtease Screening ServicesorUbiquitination Screening Services.

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78865

USP14 Inhibitor Screening Assay Kit

The USP14 Inhibitor Screening Assay Kit is a 96-well fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme USP14 (Ubiquitin specific peptidase 14) for screening and profiling applications. The kit contains enough purified USP14 protein, Ubiquitinated-AMC substrate, and assay buffer for 100 reactions.To determine the effect of an inhibitor on USP14 activity, the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases.Figure 1: Illustration of the assay principle.Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC) . In conjugated form, the energy emitted from the fluorochrome AMC is quenched. Upon proteolysis, AMC is no longer quenched and emits fluorescence with λexcitation/λemission maxima of 350/460 nm. The increase in fluorescence is proportional to the DUB activity.Need us to run inhibitor screens or profile your compounds against USP14? Check out ourProtease Screening ServicesorUbiquitination Screening Services.

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79094

USP10 Inhibitor Screening Assay Kit

The USP10 Inhibitor Screening Assay Kit is a fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme USP10 (Ubiquitin Specific Peptidase 10) for screening and profiling applications. The kit comes in a convenient 96-well format and contains enough purified recombinant human USP10 protein, Ubiquitinated-AMC substrate, and assay buffer for 100 reactions.To determine the effect of an inhibitor on USP10 activity the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU) and Machado-Josephin Domain (MJD) proteases.Figure 1: Illustration of the assay principle.Ubiquitin-AMC (Ub-AMC) is a fluorogenic substrate for ubiquitin hydrolases, based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC) . In the conjugated form the energy emitted from the fluorochrome AMC is quenched. Upon release from Ub, AMC emissions are no longer quenched and fluorescence with λexcitation/λemission maxima of 350/460 nm is emitted. The increase in fluorescence is proportional to the DUB activity.Need us to run inhibitor screens or profile your compounds against USP10? Check out ourProtease Screening ServicesorUbiquitination Screening Services.

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72048-2

ARG1 Inhibitor Screening Assay Kit

The ARG1 Inhibitor Screening Assay Kit is designed to measure ARG1 enzyme inhibition. The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 400 reactions. The ARG1 Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing thioarginine substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at λ=410-415 nm.

2017.5 €

72053

TPH1 Inhibitor Screening Assay Kit

The TPH1 Assay Kit is designed to measure TPH1 enzyme inhibition in a 96 reaction format. This fluorescence-based assay kit is especially suitable for high throughput screening applications. The procedure is quick, straightforward, and simple - just mix two solutions, incubate, add quenching solution, and measure the fluorescence.

1335 €

72054

TPH2 Inhibitor Screening Assay Kit

The TPH2 Assay Kit is designed to measure TPH2 enzyme inhibition in a 96 reaction format. This fluorescence-based assay kit is especially suitable for high throughput screening applications. The procedure is quick, straightforward, and simple - just mix two solutions, incubate, add quenching solution, and measure the fluorescence.

1335 €

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