Tissue, Total Protein, Human Tumor, Pancreas
4609.25 EUR
In Stock
quantity
Produktdetaljer
Katalognummer: 544 - MBS657286-5x1mg
Produktkategori: Företag och industri > Vetenskap och laboratorium
Gentaur
Storlek: 5x1mg
1737-01
Pancreas Tissue Lysate (Tumor)
Pancreas tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.