ABC-FC0008

RFP-Human Glomerular Microvascular Endothelial Cells

RFP-HGMVECs were selected from HGMVECs resistant to Zeocin after infected with RFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 4-5). Endothelial Growth Medium is recommended for cell culture and these cells have a minimum average population doubling levels 18 when cultured following the detailed protocol described below.

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cAP-m0008-SV40-RFP

SV40-RFP-Mouse Glomerular Microvascular Endothelial Cells

3901.13 €

cAP-m0008

Mouse Glomerular Microvascular Endothelial Cells

1051.41 €

ABC-TC3783

Human Renal Glomerular Endothelial Cells

Renal glomerular endothelial cells (GEC) are a specialized microvascular cell type involved in the regulation of glomerular ultrafiltration. They form the inner part of the filtration barrier and are involved in pathophysiological processes in the glomerulum. They constitutively synthesize bio-active molecules, to which this basal activity can be chronically augmented by various inflammatory and thrombotic agents. GEC injury exerts significant influences on the progression and repair process of glomerular disease. When the glomerular lesion is severe, angiogenesis is prevented due to endothelial cell injury, with subsequent sclerosis taking place in the impaired region. These glomerular endothelial cell injuries inevitably affect mesangial and epithelial cells and presumably modify the progression of renal disease by reciprocally interacting with them. Because of difficulties associated with the culture, cloning and propagation, the biological properties of these cells remain largely unknown. HRGEC from Gentaur Research Laboratories are isolated from human kidneys. HRGEC are cryopreserved after purification and shipped with dry ice. Each vial contains 5×10^5 cells in 1 ml volume. HRGEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM), and by the formation of microtublar structure in vitro. HRGEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRGEC are guaranteed to expand beyond 15 population doublings at the conditions specified by Gentaur Research Laboratories.

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ABC-TC3907

Monkey Kidney Glomerular Endothelial Cells

Monkey Kidney Glomerular Endothelial Cells from Gentaur are isolated from tissue of Cynomolgus Monkey. Monkey Kidney Glomerular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.Monkey Kidney Glomerular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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ABC-TC4148

Rat kidney glomerular endothelial cells

Rat kidney glomerular endothelial cells are isolated from tissue of 1-day-old neonatal Sprague-Dawley rats. Rat kidney glomerular endothelial cells are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

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