Reverse Transcriptase RT PCR Master Mix (5X)
333.30 €
In Stock
quantity
product details
Catalog number: 519 - MB1002
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 25 reactions
MB1002
Reverse Transcriptase RT PCR Master Mix (5X)
<p>Boster's Reverse Transcriptase RT PCR Master Mix, 5X contains all the reagents necessary for first‐strand cDNA synthesis without the template. The Master Mix contains proprietary Reverse Transcriptase, Ribonuclease Inhibitor, dNTPs and a finely balanced ratio of Oligo (dT)s and Random Primers. The Reverse Transcriptase catalyzes the synthesis of complementary DNA strands from single‐stranded RNA/DNA templates. The Reverse Transcriptase is a proprietary engineered version of the native reverse transcriptase enzyme from Moloney Murine Leukemia Virus.</p>_x000D__x000D_<p><strong>More about our proprietary Reverse Transcriptase:</strong> </p>_x000D_<p>The native Reverse Transcriptase has RNase H capacity and degrades mRNA. Using a series of strategic targeted mutations, our scientists successfully nullified the RNase H activity of our RT enzyme, thus preventing RNA degradation during first-strand cDNA synthesis, resulting in higher yields and increase in the achievable length of synthesized cDNA. The engineered Reverse Transcriptase also contains a fidelity‐enhancing sub-unit which ensures superior accuracy in reverse transcription. </p>_x000D__x000D_<p><strong>Other components in the Master Mix: </strong></p>_x000D__x000D_<p>The Ribonuclease Inhibitor protects RNA templates from RNases. The random primers anneal to any type of RNA as template. The Oligo (dT)s only anneal to the Poly (A) tail of mRNA.</p>
333.30 €
E2726-03
tiOptiTaq PCR Master Mix (2x)
Ready-to-use Solution containing tiOptiTaq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs
217.98 €
E2750-01
Hybrid PCR Master Mix (2x)
Ready-to-use Solution containing Hybrid DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs
48.44 €
E2750-02
Hybrid PCR Master Mix (2x)
Ready-to-use Solution containing Hybrid DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs
193.76 €
Boster's Reverse Transcriptase RT PCR Master Mix, 5X contains all the reagents necessary for first‐strand cDNA synthesis without the template. The Master Mix contains proprietary Reverse Transcriptase, Ribonuclease Inhibitor, dNTPs and a finely balanced ratio of Oligo (dT)s and Random Primers. The Reverse Transcriptase catalyzes the synthesis of complementary DNA strands from single‐stranded RNA/DNA templates. The Reverse Transcriptase is a proprietary engineered version of the native reverse transcriptase enzyme from Moloney Murine Leukemia Virus.
_x000D__x000D_More about our proprietary Reverse Transcriptase:
_x000D_The native Reverse Transcriptase has RNase H capacity and degrades mRNA. Using a series of strategic targeted mutations, our scientists successfully nullified the RNase H activity of our RT enzyme, thus preventing RNA degradation during first-strand cDNA synthesis, resulting in higher yields and increase in the achievable length of synthesized cDNA. The engineered Reverse Transcriptase also contains a fidelity‐enhancing sub-unit which ensures superior accuracy in reverse transcription.
_x000D__x000D_Other components in the Master Mix:
_x000D__x000D_The Ribonuclease Inhibitor protects RNA templates from RNases. The random primers anneal to any type of RNA as template. The Oligo (dT)s only anneal to the Poly (A) tail of mRNA.