Human IL-2, also known as interleukin 2, (Genbank Accession No. NM_000586), a.a. 21-153(end), with C145A mutation, expressed in an E.coli expression system. MW = 15 kDa.
This subline of the EL4 cell line is capable of producing IL-2 in response to phorbol-12-myristate-13-acetate (PMA). Following stimulation of EL4.IL2 using PMA for 24 hours up to 2500 units/ml of IL-2 may be produced.