Rat 25-OH-D (25 Hydroxy Vitamin D) ELISA Kit

ELK0843-48T

Rat25-OH-D(25HydroxyVitaminD)ELISAKit

Competitive Inhibition

966.5 €

ELK0843-96T

Rat25-OH-D(25HydroxyVitaminD)ELISAKit

Competitive Inhibition

1049 €

ELK0875-48T

Rat 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 25-OH-D protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.

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