CSI094Mu01

Primary Mouse Splenic Fibroblasts (SF)

1507.93 €

CSI094Ca01

Primary Canine Splenic Fibroblasts (SF)

1538.23 €

ABC-TC3818

Human Splenic Fibroblasts

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. They secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. There is good evidence that fibroblasts in different parts of the body are intrinsically different and display different regulatory functions on surrounding cells. For example, splenic fibroblasts have been shown to regulate the development of NK cells from blood progenitors through an expression of bioactive IL-15. HSF from Gentaur Research Laboratories are isolated from human spleen tissue. HSF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HSF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

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ABC-TC1344

Mouse Breast Carcinoma Associated Fibroblasts

Mouse Carcinoma Associated Fibroblasts are isolated from human xenograft of pathogen-free laboratory nude mice. Human breast adenocarcinoma cells (MDA-MB-231) were subcutaneously injected into the nude mice for 4-6 weeks and the tumor tissues were then harvested for cell culture.

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ABC-TC3040

B6129SF2/J Mouse Cardiac Fibroblasts

Mouse cardiac fibroblasts are isolated from tissue of pathogen-free laboratory mice. Mouse cardiac fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 0.5 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.

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ABC-TC3041

B6129SF2/J Mouse Embryonic Fibroblasts

Mouse Embryonic Fibroblasts are isolated from day 13 mouse embryos of pathogen-free laboratory mice. They are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 0.5 hour and incubated in Culture Complete Growth Medium for 3-7 days.

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