Primary Mouse Splenic Fibroblasts (SF)
1507.93 €
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Catalog number: 338 - CSI094Mu01
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: 5X10^5
ABC-TC3083
BALB/c Mouse Liver Fibroblasts
Mouse liver fibroblasts are isolated from tissue of pathogen-free laboratory mice. Mouse liver fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.
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ABC-TC3087
BALB/c Mouse Lung Fibroblasts
Mouse lung fibroblasts are isolated from tissue of pathogen-free laboratory mice. Mouse lung fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium generally for 3-7 day.
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ABC-TC3089
BALB/c Mouse Lymphatic Fibroblasts
BALB/c Mouse Primary Lymphatic Fibroblasts from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Lymphatic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Primary Lymphatic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.