F5C2 can be used as epitope-defined marker in germ cell tumors and is applicable on frozen sections directly as well as on paraffin sections after a heat induced antigen retrieval step. F5C2 can be used at least as tracer antibody in ELISA to detect PLAP in serum of S, F, FS and I phenotypes. F5C2 can also be used for immunosorbent purification and for radioimmuno-localization.