T4652

Mouse Primary Ovarian Microvascular Endothelial Cells

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T4197

Human Primary Ovarian Microvascular Endothelial Cells

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ABC-TC4188

Rat Ovarian Microvascular Endothelial Cells

Rat Ovarian Microvascular Endothelial Cells from Gentaur are isolated from tissue of adult Sprague-Dawley Rats. Rat Ovarian Microvascular Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.Rat Ovarian Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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ABC-TC3479

Human Adrenal Microvascular Endothelial Cells

The adrenal gland plays an essential role in regulating homeostasis in the body through the secretion of corticosteroid and androgen hormones. The secreted steroids arise from three zones that form the adrenal gland: the zona glomerulosa, zona fasciculate, and zona reticularis. Microvascular endothelial cells line the blood vessels and contribute to many biological processes such as hemostasis, coagulation, trafficking of lymphocytes, and the inflammatory response. Microvascular endothelial cells (MEC) are of importance in the adrenal gland for their regulatory function on the secretion of adrenal hormones. Studies have shown that factors secreted from HAdMEC, such as endothelin-1, nitric oxide, adrenomedullin and PAMP affect aldosterone secretion. HAdMEC differ from MECs from different organs in function and phenotype. For example, compared to brain MEC and liver MEC, adrenal MEC are highly resistant to glucocorticoid-induced cytotoxicity showing that MECs are specialized to the tissues they belong to. HAdMEC from Gentaur Research Laboratories are isolated from human adrenal tissue. HAdMEC are cryopreserved at passage one and delivered frozen. Each vial contains 5×10 5 cells in 1 ml volume. HAdMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CED31 (P-CAM). HAdMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAdMEC are guaranteed to further culture for 15 population doublings in the conditions provided by Gentaur Research Laboratories

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ABC-TC3513

Human Bladder Microvascular Endothelial Cells

The walls of capillaries are composed of a single layer of microvascular endothe-lial cells. These cells differ in morphology and other properties depending on the tissues the capillaries supply. Therefore, Gentaur offers a range of Microvascular Endothelial Cells produced at Gentaur's cell culture facility from normal human tissues of different origins.Shortly after isolation, all Gentaur Microvascular Endothelial Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

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ABC-TC3524

Human Brain Microvascular Endothelial Cells

Human Brain Microvascular Endothelial Cells were initiated by elutriation of dispase dissociated normal human brain cortex tissue.These cells were originated using Complete Serum-Free Medium Kit, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells.

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