Mouse Lung Tissue Preparation Buffer 2: Normal Lung Fibroblasts
493.27 EUR
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Produktdetaljer
Katalognummer: 567 - 9-80179
Produktkategori: Företag och industri > Vetenskap och laboratorium
Gentaur
Storlek: 1 x 100 ml
1701-05
Lung Tissue Lysate (Normal)
Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
521.05 €