Catalog number: 10041 - A5006
Product Category: Business & Industrial > Science & Laboratory
Size: 100ul X 3
A5006
A recombinant monoclonal antibody from rabbit against human Met (c-Met) for WB, IF, IHC,ELISA
CSB-RA013714MA1HU-100ul
Overlay Peak curve showing Hela cells stained with CSB-RA013714MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10<sup>6</sup>cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
CSB-RA013714MA1HU-50ul
Overlay Peak curve showing Hela cells stained with CSB-RA013714MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10<sup>6</sup>cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
CSB-RA634199A0HU-100ul
IHC image of CSB-RA634199A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.