Transformed from HEK293 cells by lentiviral system with sequence verified firefly luciferase gene II and CRE recombinase. Luciferase and CRE was co-expressed under CMV promoter as seperated protein(not as fusion). The GFP-Blasticidin fusin dual marker was expressed under Rsv promoter. The cell constitutively co-expresses both firefly Luciferase and CRE.
Recombinant clonal stable CHO cell line constitutively expressing full length human CD19 protein (also known as B4 or CVID3, Genbank #NM_001770) and the firefly luciferase. Surface expression of CD19 was confirmed by flow cytometry.
Transformed from HEK293 cells, expressing the firefly luciferase gene. Luciferase was expressed under CMV promoter, A Neomycin antibiotic marker was expressed under Rsv promoter. The cell constitutively express Luciferase enzyme.