Pre-made lentiviral particles bicistronically express (under EF1a promoter) nuclear permeable CRE recombinase and the RFP marker under the same suEF1a promoter. A Blasticidin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.
Lentivirus express "LoxP-GFP-stop-LoxP-RFP-Stop" cassette under EF1a promoter, containing a Blasticidin antibiotic selection. Lentivirus is concentrated and provied in PBS solution.