LoxP GFP/RFP ColorSwitch (EF1a, Neo) lentivirus
970 EUR
In Stock
quantity
Produktdetaljer
Katalognummer: 500 - LVP1332-Neo-PBS
Produktkategori: Företag och industri > Vetenskap och laboratorium
Gentaur
Storlek: 1x10^8 IFU/ml x 200ul
Parameters
| Storage and shipping | (-80oC) |
|---|---|
| Storage and shipping | With |
ABC-RC128D
loxP-GFP-RFP(Neo) Report Cell Line-HEK293
The CRE reporting cell lines are used to monitor or confirm the efficiency of CRE recombination in vivo. It is a great method and easy tool to verify the performance of your CRE enzyme(your CRE expression plasmids, or pre-made CRE expression lentivirus, or purified CRE enzyme) in vivo conditions. It is also a control test to verify your CRE-loxP based system. Transformed from HEK293 cells, expressing "LoxP-GFP-stop-LoxP-RFP" cassette under CMV promoter(see Color-Switch cassette below). Cell line also contains a Neomycin antibiotic marker under RSV promoter.The cell line demonstrates strong GFP fluorescent signal in normal culture condition as the constitutive CMV promoter drives the GFP high expression to the stop codon. The downstream RFP ORF was not expressed because of the stop codon after the GFP ORF. Once the CRE protein was present in nuclear, the CRE excises/deletes the DNA fragment between two loxP sites, which removes the stop codon(see the DNA structure scheme above). As a result, the RFP ORF is then expressed under the CMV promoter, and the cell line switches to RFP fluorescent. The RFP signal can be easily monitored via fluorescent cell sorting(for the ratio between GFP and RFP cells), or visualized under microscope, or measured the fluorescent intensity by a meter or reader with RFP filter set.
Ask for quotation
ABC-RC127D
loxP-GFP-RFP(Bsd) Report Cell Line-HEK293
The CRE reporting cell lines are used to monitor or confirm the efficiency of CRE recombination in vivo. It is a great method and easy tool to verify the performance of your CRE enzyme(your CRE expression plasmids, or pre-made CRE expression lentivirus, or purified CRE enzyme) in vivo conditions. It is also a control test to verify your CRE-loxP based system. Transformed from HEK293 cells, expressing "LoxP-GFP-stop-LoxP-RFP" cassette under CMV promoter(see Color-Switch cassette below). Cell line also contains a blasticidin antibiotic marker under RSV promoter.The cell line demonstrates strong GFP fluorescent signal in normal culture condition as the constitutive CMV promoter drives the GFP high expression to the stop codon. The downstream RFP ORF was not expressed because of the stop codon after the GFP ORF. Once the CRE protein was present in nuclear, the CRE excises/deletes the DNA fragment between two loxP sites, which removes the stop codon(see the DNA structure scheme above). As a result, the RFP ORF is then expressed under the CMV promoter, and the cell line switches to RFP fluorescent. The RFP signal can be easily monitored via fluorescent cell sorting(for the ratio between GFP and RFP cells), or visualized under microscope, or measured the fluorescent intensity by a meter or reader with RFP filter set.
Ask for quotation
ABC-RC129D
loxP-GFP-RFP(Puro) Report Cell Line-HEK293
The CRE reporting cell lines are used to monitor or confirm the efficiency of CRE recombination in vivo. It is a great method and easy tool to verify the performance of your CRE enzyme(your CRE expression plasmids, or pre-made CRE expression lentivirus, or purified CRE enzyme) in vivo conditions. It is also a control test to verify your CRE-loxP based system. Transformed from HEK293 cells, expressing "LoxP-GFP-stop-LoxP-RFP" cassette under CMV promoter(see Color-Switch cassette below). Cell line also contains a Puromycin antibiotic marker under RSV promoter.The cell line demonstrates strong GFP fluorescent signal in normal culture condition as the constitutive CMV promoter drives the GFP high expression to the stop codon. The downstream RFP ORF was not expressed because of the stop codon after the GFP ORF. Once the CRE protein was present in nuclear, the CRE excises/deletes the DNA fragment between two loxP sites, which removes the stop codon(see the DNA structure scheme above). As a result, the RFP ORF is then expressed under the CMV promoter, and the cell line switches to RFP fluorescent. The RFP signal can be easily monitored via fluorescent cell sorting(for the ratio between GFP and RFP cells), or visualized under microscope, or measured the fluorescent intensity by a meter or reader with RFP filter set.
Ask for quotation
LVP1262
ER-SEAP (Neo) Lentivirus
Lentivirus express SEAP reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
649.75 €
LVP1266
G_RE-SEAP (Neo) Lentivirus
Pre-made lentivirus express SEAP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
649.75 €
LVP1270
Gli-SEAP (Neo) Lentivirus
Pre-made lentivirus express SEAP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
649.75 €
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