Immortalized Mouse Embryonic Cystic Fibrosis Fibroblasts-SV40
4241 EUR
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quantity
Produktdetaljer
Katalognummer: 10021 - T0257
Produktkategori: Företag och industri > Vetenskap och laboratorium
Gentaur
Storlek: 1x106 cells / 1.0 ml
ABC-SC0101T
HighQC™ C57BL/6 Mouse Embryonic Fibroblasts
C57BL/6 Mouse Embryonic Fibroblasts from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Embryonic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 2x10^6 cells per ml and is delivered frozen. Mouse Embryonic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 (Millipore USA) by immunofluorescence staining. Cells can be expanded for 3-6 passages at a split ratio of 1: 2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.
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ABC-SC0102T
HighQC™ INOS Knockout Mouse Embryonic Fibroblasts
Mouse Embryonic Fibroblasts from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Embryonic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Embryonic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1: 2 or 1: 3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.