Human Primary Pancreatic Fibroblasts are isolated from normal human pancreatic tissue. Human Primary Pancreatic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml.
Human Dermal Fibroblasts-Adult are isolated from adult human skin. Human Dermal Fibroblasts-Adult are cryopreserved at P0 and delivered frozen. Human Dermal Fibroblasts-Adult are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. Human Dermal Fibroblasts-Adult are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.Human Dermal Fibroblasts provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology. Fibroblasts are cryopreserved as primary cells to ensure the highest viability and plating efficiency.
Human Cardiac-Related Fibroblasts include Aortic Adventitial Fibroblasts (AoAF) isolated from the tunica external of the ascending or descending aorta and cardiac fibroblasts (NHCF-A and -V) isolated from the atrium or verticle.
Fibroblasts are the most common cells of mammalian connective tissue. AcceGen offers an Immortalised fibroblast cell line originated from the human foreskin.