Immortalized Human Carotid Artery Smooth Muscle Cells-SV40
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Catalog number: 566 - CSC-I9070L
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: One Frozen vial
ABC-TC3550
Human Carotid Artery Smooth Muscle Cells
Human Carotid Artery Smooth Muscle Cells are primary smooth muscle cells isolated from normal healthy human normal carotid artery and are cryopreserved at two passage. HCtASMC can be propagated at least 10 doublings.
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ABC-TC3235
C57BL/6 Mouse Pulmonary Artery Smooth Muscle Cells
Mouse Pulmonary Vein Vascular Smooth Muscle Cells from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Pulmonary Vein Vascular Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Pulmonary Vein Vascular Smooth Muscle Cells are characterized by immunofluorescent cell staining with antibodies of α-smooth muscle actin and are negative for bacteria, yeast, fungi, and mycoplasma.Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur.Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.