Immortalized Canine Coronary Artery Smooth Muscle Cells-SV40
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Catalog number: 566 - CSC-I2138Z
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: One Frozen vial
ABC-TC4031
Porcine Coronary Artery Smooth Muscle Cells
Porcine large artery smooth muscle cells are derived from tunica intima and tunica media of healthy, fibrous plaque-free porcine arteries. They are cryopreserved at first passage (PCASMC at second passage) and can be cultured and propagated at least 16 passages.
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ABC-TC3167
C57BL/6 Mouse Artery Smooth Muscle Cells
Mouse Artery Vascular Smooth Muscle Cells from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Artery Vascular Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Artery Vascular Smooth Muscle Cells are characterized by immunofluorescent cell staining with antibodies of α-smooth muscle actin and are negative for bacteria, yeast, fungi, and mycoplasma.Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur.Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.