Bovine Brain Microvascular Endothelial Cells (BBMVEC) are isolated from microvessels of bovine brain. The cells are cryopreserved at second passage. BBMVEC can be cultured and propagated for at least 12 population doublings.
Human Brain Microvascular Endothelial Cells were initiated by elutriation of dispase dissociated normal human brain cortex tissue.These cells were originated using Complete Serum-Free Medium Kit, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells.