Human Thyroid Fibroblasts
Ask for quotation
On request
quantity
product details
Catalog number: 566 - CSC-7702W
Product Category: Business & Industrial > Science & Laboratory
Gentaur
Size: One Frozen vial
ABC-TC4232
Rat Thyroid Fibroblasts
Rat Primary Thyroid Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Thyroid Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Thyroid Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
Ask for quotation
ABC-TC3832
Human Thyroid Fibroblasts
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. Because the thyroid is easily susceptible to inflammatory autoimmune diseases that can result in tissue damage, thyroid fibroblasts may play an important role in regulating thyroid function and reaction to disease. HThF from Gentaur Research Laboratories are isolated from human thyroid tissue. HThF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HThF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HThF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HThF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.
Ask for quotation
ABC-TC4064
Rabbit Lung Fibroblasts
Rabbit Primary Lung Fibroblasts from Gentaur are isolated from lung tissue of New Zealand White Rabbits. Rabbit Primary Lung Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Rabbit Primary Lung Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
Ask for quotation
ABC-TC4070
Rat Aortic Fibroblasts
Rat Primary Aortic Fibroblasts from Gentaur are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Aortic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Aortic Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.