ABC-HP021X

Human Stomach Epithelial Cells

Human Stomach Epithelial Cells are isolated from normal human stomach tissue. Cells are cryopreserved at passage 3 and delivered frozen.

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ABC-TC4228

Rat Stomach Epithelial Cells

Rat Stomach Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Stomach Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Stomach Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Stomach Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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CSC-C9381W

Human Stomach Epithelial Cells

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ABC-TC4233

Rat Tracheal Epithelial Cells

Rat Primary Tracheal Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Primary Tracheal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Primary Tracheal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Primary Tracheal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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ABC-TC4284

Rat Bronchial Epithelial Cells

Rat Primary Bronchial Epithelial Cells from Gentaur are isolated from bronchial tissue of 6-8 week old laboratory Sprague–Dawley rat. Rat Primary Bronchial Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur’ Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 0.5×10^6 cells and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.

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ABC-TC4365

Human Oral Epithelial Cells

Cells are only guaranteed with purchase of Gentaur Media, Extra Cellular Matrix, Trypsin EDTA, 1X PBS, and freezing media for the appropriate cell culture, for 30 days from the date of shipment.

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