Catalog number: 10021 - HS0020
Product Category: Business & Industrial > Science & Laboratory
Size: 6 Slides/Pack
ST1451a
Stomach cancer, matched NAT, matched metastasis tissue (recent year collection samples), including pathology grade, TNM and clinical stage, 71 cases/145 cores, replacing ST1451
ST1401a
Stomach cancer and stomach tissue array, including pathology grade, TNM and clinical stage, with IHC result Her2, 140 cases/140 cores, replacing ST1401
1475
Stomach tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.