ABC-TC3818

Human Splenic Fibroblasts

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. They secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. There is good evidence that fibroblasts in different parts of the body are intrinsically different and display different regulatory functions on surrounding cells. For example, splenic fibroblasts have been shown to regulate the development of NK cells from blood progenitors through an expression of bioactive IL-15. HSF from Gentaur Research Laboratories are isolated from human spleen tissue. HSF are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HSF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

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CSC-C9230J

Human Splenic Fibroblasts 

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ABC-TC3052

B6129SF2/J Mouse Splenic Fibroblasts

Mouse splenic fibroblasts are isolated from tissue of pathogen-free laboratory mice. Mouse splenic fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 0.5 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.

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ABC-TC3835

Human Tonsil Fibroblasts

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. Fibroblasts are among the most accessible normal mammalian cell types and still the most amenable to culture in vitro.HTF from Gentaur Research Laboratories are isolated from human tonsils. HTF are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HTF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HTF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTF are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

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ABC-TC3863

Human Uterine Fibroblasts

Derived from the mesoderm, connective tissue is involved in maintaining the structural integrity of the body. The predominant cells found in connective tissue are fibroblasts, which continuously secrete various components of the extracellular matrix. Depending on the site of origin and physiological status, fibroblasts may exhibit different morphological phenotypes and various functional properties.Gentaur offers a range of Human Fibroblasts produced at Gentaur's cell culture facility. The cells are isolated from normal human juvenile foreskin or adult skin from different locations. In addition, Gentaur offers Fibroblasts from adult lung tissue, aortic adventitial tissue, and villous placental tissue.Shortly after isolation, all Gentaur Human Fibroblasts are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium,Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing. Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

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ABC-TC3903

Monkey Aortic Fibroblasts

Monkey Primary Aortic Fibroblasts from Gentaur are isolated from aortic tissue of Cynomolgus Monkey. Monkey Primary Aortic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Monkey Primary Aortic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

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