Catalog number: 994 - ALHE46
Product Category: Business & Industrial > Science & Laboratory
Size: 1ml frozen Vial
ABC-TC3779
Primary Human Lung Microvascular Endothelial Cells(Human Pulmonary Microvascular Endothelial Cells) are initiated from normal human peripheral lung tissue.These cells were originated using Complete Serum-Free Medium Kit With AcceSup™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm1 flask.
ABC-TC4203
Rat Pulmonary Artery Smooth Muscle Cells from Gentaur are isolated from tissue of Sprague-Dawley Rats. Rat Pulmonary Artery Smooth Muscle Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Cells are negative for bacteria, yeast, fungi, and mycoplasma and are characterized by immunofluorescent staining with antibodies to α-smooth muscle actin. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur.Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with smooth muscle cell cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
ABC-TC3134
Bovine large artery smooth muscle cells are derived from tunica intima and tunica media of healthy, fibrous, plaque-free bovine large arteries. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings.