Catalog number: 994 - ALHE25
Product Category: Business & Industrial > Science & Laboratory
Size: 1ml frozen Vial
ABC-TC4197
Rat Prostate Epithelial Cells from Gentaur are isolated from tissue of adult laboratory Sprague–Dawley Rats. Rat Prostate Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Prostate Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Prostate Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
ABC-TC3995
Human Prostate Epithelial Cells (HPrEC), when grown in LIPro Medium, provides an ideal serum-free culture model, without retinoic acid, for the study of prostate cells. Common uses for HPrEC are studies of hormonal regulation of the prostate, regulation and control of the secretory function of prostate cells and as a control for the study of prostate cancer. HPrEC are cryopreserved as secondary cells to ensure the highest viability and plating efficiency. Our HPrEC are quality tested in LIPro Medium to ensure optimal serum-free growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented media. Cell Features:HPrEC are cryopreserved as secondary cells, e.g. cells are isolated fromhuman prostate tissue and expanded twice in culture vessels before beingharvested for cryopreservation.HPrEC proliferate in a serum-free, retinoic acid-free medium withoutphenol red or antimicrobials when cultured in ProstaLifeTM medium.HPrEC are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality.